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Streptavidin Separopore® 4B

 

MSRP: $629.74*

Price: $538.24*

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Applications:

  • Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
  • The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
  • The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications.
  • The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
  • Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.


Catalog No.:20181037-3 ( 506916 )
Size:10 mL
Brand Name:bioPLUS™, Separopore®
Hazmat Shipping:Non-hazardous
Shelf life:2 years
Storage:2-8°C
Matrix:Separopore 4B (agarose beads, 4%)
Matrix Activation:Cyanogen bromide
Particle Size Range:53 -180 μm
Binding Capacity:>20 μg biotin / ml drained gel
Suspension:Supplied as suspension in buffered saline solution with 0.05% sodium azide.
pH Stability:3 - 10
Binding Wash Buffer:50 mM ammonium carbonate, 0.5 M NaCl, pH 10. Elution buffer: 50 mM ammonium acetate, 0.5 M NaCl, pH 4
Ligand:Native Streptavidin (from Streptomyces avidinii)

*Cold Shipping and packing charges apply.

  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


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