Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding site. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme substrate calmoldulin-Ca2+ complexes are particularly stable. Used for IP studies and bulk purification of CAM-binding proteins.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
|Catalog No.||:||20181001-2 ( 506461 )
||Brand Name||:||bioPLUS™, Separopore®
||Shelf life||:||2 years
||Matrix||:||Separopore® 4B (agarose beads, 4 %)
||Matrix Activation||:||Cyanogen bromide
||Particle Size Range||:||53 - 180 μm
||Spacer Arm||:||1 atom
||Suspension||:||Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
||pH Stability||:||4 - 9
||Chemical Stability||:||Stable to all commonly used aqueous solutions
||Physical Stability||:||Negligible volume variation due to changes in pH or ionic strength.