Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Separopore® 4B provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione Separopore® 4B is prepared by covalently coupling glutathione to epoxy-activated 4 % crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel.. Bound GST –fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
||Brand Name||:||bioPLUS™, Separopore®
||Shelf life||:||2 years
||Appearance Form||:||Gel Suspension
||Matrix||:||Separopore® 4B (agarose beads, 4%)
||Particle Size Range||:||52 - 165 μm
||Spacer Arm||:||12 atoms
||Binding Capacity||:||5 - 10 mg recombinant GST-tagged protein / ml drained gel
||Suspension||:||Supplied as suspension in 20% ethanol
||pH Stability||:||4 - 13